trim46, rabbit, wb Search Results


90
ABclonal Biotechnology 5-116trim25 pab
5 116trim25 Pab, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bethyl suppliers atr wb 13934t cst p atr wb 2853t cst chk1 wb 2360t cst p chk1 wb 2348t cst rpa32 wb 2208t cst
Suppliers Atr Wb 13934t Cst P Atr Wb 2853t Cst Chk1 Wb 2360t Cst P Chk1 Wb 2348t Cst Rpa32 Wb 2208t Cst, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ABclonal Biotechnology hpd-l1 wb; n-terminal
Hpd L1 Wb; N Terminal, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vicam Inc dontesttm wide bore (wb) immunoaffinity columns
Dontesttm Wide Bore (Wb) Immunoaffinity Columns, supplied by Vicam Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vicam Inc aflatesttm wb immunoaffinity columns
Aflatesttm Wb Immunoaffinity Columns, supplied by Vicam Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GeneTex hpd-l1 wb; c-terminal
Hpd L1 Wb; C Terminal, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson e-cadherin
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p62  (Abnova)
90
Abnova p62
(A) HeLa cells were transfected with the indicated siRNAs for 48 h and lysates were immunoblotted against LAMP1 and loading control GAPDH. (B) HeLa cells transfected with control (Ctrl) and SMPD2 siRNA for 48 h were stained with Lysotracker and DAPI and analyzed by confocal microscopy. (C) Quantifications of Lysotracker puncta per cell from (B). Unpaired Student’s two-tailed t-test . (D) HeLa cells transfected with the indicated siRNAs for 48 h were then grown in FBS containing medium or serum-starved (starv) with or without Bafilomycin A (Baf) for 16 h. (E) Quantifications of lysosomal turnover of <t>P62</t> and LC3B-II under basal conditions from (D). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test, not significant. (F) Quantifications of lysosomal turnover of P62 and LC3B-II under serum starvation conditions from (D). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. (G) HeLa cells transfected with the indicated siRNA for 48 h were then treated with DMSO or MG132 for 16 h. Lysates were immunoblotted against LAMP1, BiP, β-Catenin, and loading control GAPDH. (H) Real-time qPCR analysis of SMPD2 mRNA in HeLa cells after 48 h of SMPD2 KD. Unpaired Student’s two-tailed t-test , ****p < 0.0001. (I) Realtime qPCR analysis of LAMP1 mRNA in HeLa cells after 48 h of SMPD2 KD. Unpaired Student’s two-tailed t-test , ****p < 0.0001. All the experiments from (A)-(I) were done in at least three biological replicates.
P62, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ABclonal Biotechnology p38
(A) HeLa cells were transfected with the indicated siRNAs for 48 h and lysates were immunoblotted against LAMP1 and loading control GAPDH. (B) HeLa cells transfected with control (Ctrl) and SMPD2 siRNA for 48 h were stained with Lysotracker and DAPI and analyzed by confocal microscopy. (C) Quantifications of Lysotracker puncta per cell from (B). Unpaired Student’s two-tailed t-test . (D) HeLa cells transfected with the indicated siRNAs for 48 h were then grown in FBS containing medium or serum-starved (starv) with or without Bafilomycin A (Baf) for 16 h. (E) Quantifications of lysosomal turnover of <t>P62</t> and LC3B-II under basal conditions from (D). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test, not significant. (F) Quantifications of lysosomal turnover of P62 and LC3B-II under serum starvation conditions from (D). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. (G) HeLa cells transfected with the indicated siRNA for 48 h were then treated with DMSO or MG132 for 16 h. Lysates were immunoblotted against LAMP1, BiP, β-Catenin, and loading control GAPDH. (H) Real-time qPCR analysis of SMPD2 mRNA in HeLa cells after 48 h of SMPD2 KD. Unpaired Student’s two-tailed t-test , ****p < 0.0001. (I) Realtime qPCR analysis of LAMP1 mRNA in HeLa cells after 48 h of SMPD2 KD. Unpaired Student’s two-tailed t-test , ****p < 0.0001. All the experiments from (A)-(I) were done in at least three biological replicates.
P38, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trim46%2C+rabbit%2C+wb/pmc08983694__41420_2022_992_MOESM1_ESM-25-42-41?v=ABclonal+Biotechnology
Average 90 stars, based on 1 article reviews
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Bio-Techne corporation human trailr1/tnfrsf10a antibody
(A) HeLa cells were transfected with the indicated siRNAs for 48 h and lysates were immunoblotted against LAMP1 and loading control GAPDH. (B) HeLa cells transfected with control (Ctrl) and SMPD2 siRNA for 48 h were stained with Lysotracker and DAPI and analyzed by confocal microscopy. (C) Quantifications of Lysotracker puncta per cell from (B). Unpaired Student’s two-tailed t-test . (D) HeLa cells transfected with the indicated siRNAs for 48 h were then grown in FBS containing medium or serum-starved (starv) with or without Bafilomycin A (Baf) for 16 h. (E) Quantifications of lysosomal turnover of <t>P62</t> and LC3B-II under basal conditions from (D). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test, not significant. (F) Quantifications of lysosomal turnover of P62 and LC3B-II under serum starvation conditions from (D). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. (G) HeLa cells transfected with the indicated siRNA for 48 h were then treated with DMSO or MG132 for 16 h. Lysates were immunoblotted against LAMP1, BiP, β-Catenin, and loading control GAPDH. (H) Real-time qPCR analysis of SMPD2 mRNA in HeLa cells after 48 h of SMPD2 KD. Unpaired Student’s two-tailed t-test , ****p < 0.0001. (I) Realtime qPCR analysis of LAMP1 mRNA in HeLa cells after 48 h of SMPD2 KD. Unpaired Student’s two-tailed t-test , ****p < 0.0001. All the experiments from (A)-(I) were done in at least three biological replicates.
Human Trailr1/Tnfrsf10a Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trim46%2C+rabbit%2C+wb/custom%40mab347%40henry_ryan_edward__2012__novel_molecular_mechanisms_that_modulate_the_cellular_response_to_p53_activation?v=Bio-Techne+corporation
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99
ATCC issatchenkia orientalis kudrjanzev
(A) HeLa cells were transfected with the indicated siRNAs for 48 h and lysates were immunoblotted against LAMP1 and loading control GAPDH. (B) HeLa cells transfected with control (Ctrl) and SMPD2 siRNA for 48 h were stained with Lysotracker and DAPI and analyzed by confocal microscopy. (C) Quantifications of Lysotracker puncta per cell from (B). Unpaired Student’s two-tailed t-test . (D) HeLa cells transfected with the indicated siRNAs for 48 h were then grown in FBS containing medium or serum-starved (starv) with or without Bafilomycin A (Baf) for 16 h. (E) Quantifications of lysosomal turnover of <t>P62</t> and LC3B-II under basal conditions from (D). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test, not significant. (F) Quantifications of lysosomal turnover of P62 and LC3B-II under serum starvation conditions from (D). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. (G) HeLa cells transfected with the indicated siRNA for 48 h were then treated with DMSO or MG132 for 16 h. Lysates were immunoblotted against LAMP1, BiP, β-Catenin, and loading control GAPDH. (H) Real-time qPCR analysis of SMPD2 mRNA in HeLa cells after 48 h of SMPD2 KD. Unpaired Student’s two-tailed t-test , ****p < 0.0001. (I) Realtime qPCR analysis of LAMP1 mRNA in HeLa cells after 48 h of SMPD2 KD. Unpaired Student’s two-tailed t-test , ****p < 0.0001. All the experiments from (A)-(I) were done in at least three biological replicates.
Issatchenkia Orientalis Kudrjanzev, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trim46%2C+rabbit%2C+wb/custom%406258%4026974150?v=ATCC
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Image Search Results


(A) HeLa cells were transfected with the indicated siRNAs for 48 h and lysates were immunoblotted against LAMP1 and loading control GAPDH. (B) HeLa cells transfected with control (Ctrl) and SMPD2 siRNA for 48 h were stained with Lysotracker and DAPI and analyzed by confocal microscopy. (C) Quantifications of Lysotracker puncta per cell from (B). Unpaired Student’s two-tailed t-test . (D) HeLa cells transfected with the indicated siRNAs for 48 h were then grown in FBS containing medium or serum-starved (starv) with or without Bafilomycin A (Baf) for 16 h. (E) Quantifications of lysosomal turnover of P62 and LC3B-II under basal conditions from (D). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test, not significant. (F) Quantifications of lysosomal turnover of P62 and LC3B-II under serum starvation conditions from (D). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. (G) HeLa cells transfected with the indicated siRNA for 48 h were then treated with DMSO or MG132 for 16 h. Lysates were immunoblotted against LAMP1, BiP, β-Catenin, and loading control GAPDH. (H) Real-time qPCR analysis of SMPD2 mRNA in HeLa cells after 48 h of SMPD2 KD. Unpaired Student’s two-tailed t-test , ****p < 0.0001. (I) Realtime qPCR analysis of LAMP1 mRNA in HeLa cells after 48 h of SMPD2 KD. Unpaired Student’s two-tailed t-test , ****p < 0.0001. All the experiments from (A)-(I) were done in at least three biological replicates.

Journal: bioRxiv

Article Title: Neutral sphingomyelinase 1 regulates cellular fitness at the level of ER stress and cell cycle

doi: 10.1101/2022.02.23.481585

Figure Lengend Snippet: (A) HeLa cells were transfected with the indicated siRNAs for 48 h and lysates were immunoblotted against LAMP1 and loading control GAPDH. (B) HeLa cells transfected with control (Ctrl) and SMPD2 siRNA for 48 h were stained with Lysotracker and DAPI and analyzed by confocal microscopy. (C) Quantifications of Lysotracker puncta per cell from (B). Unpaired Student’s two-tailed t-test . (D) HeLa cells transfected with the indicated siRNAs for 48 h were then grown in FBS containing medium or serum-starved (starv) with or without Bafilomycin A (Baf) for 16 h. (E) Quantifications of lysosomal turnover of P62 and LC3B-II under basal conditions from (D). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test, not significant. (F) Quantifications of lysosomal turnover of P62 and LC3B-II under serum starvation conditions from (D). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. (G) HeLa cells transfected with the indicated siRNA for 48 h were then treated with DMSO or MG132 for 16 h. Lysates were immunoblotted against LAMP1, BiP, β-Catenin, and loading control GAPDH. (H) Real-time qPCR analysis of SMPD2 mRNA in HeLa cells after 48 h of SMPD2 KD. Unpaired Student’s two-tailed t-test , ****p < 0.0001. (I) Realtime qPCR analysis of LAMP1 mRNA in HeLa cells after 48 h of SMPD2 KD. Unpaired Student’s two-tailed t-test , ****p < 0.0001. All the experiments from (A)-(I) were done in at least three biological replicates.

Article Snippet: The following antibodies and dilutions were used for western blot (WB) or for immunofluorescence staining (IF): LAMP1 (WB 1:1000, Abcam, ab24170); Syntenin (WB 1:1000, Abcam, ab133267); GAPDH (WB 1:1000, Millipore, CB1001); Calnexin (WB 1:1000, Abcam, ab75801), P62 (WB 1:1000, Abnova); LC3B (WB 1:1000, Santa Cruz); Ceramide (IF 1:100, Enzo, ALX-804-196); BiP (WB 1:1000, CST), nSMase1 (IF 1:100, WB 1:1000, Sigma); β-Catenin (WB 1:1000, BD Transduction), PDI (WB 1:1000, CST), FL-Caspase 3 (WB 1:1000, CST); C-Caspase-3 (WB 1:100, CST), FL-Caspase 7 (WB 1:1000, CST); C-Caspase-7 (WB 1:1000); FL-PARP (WB 1:1000, CST), C-PARP (WB 1:1000, CST), p-CHk2 (Thr68) (WB 1:1000, CST); p-Rb (Ser807/811) (WB 1:1000, CST); p-Chk1 (Ser345) (WB 1:1000, CST); p-AKT (Ser473) (WB 1:1000, CST); Puromycin (WB 1:1000, Millipore);; pPTEN (Ser380) (WB 1:1000, CST)

Techniques: Transfection, Control, Staining, Confocal Microscopy, Two Tailed Test